FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13546549
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC1633471.205820021025281TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA201800.042.7931981
ATCGGAA204600.042.2034262
TCGGAAG204650.042.1051643
CGTCTGA203850.041.4978416
CGGAAGA207400.041.4709434
ACACGTC204000.041.46732313
TCTCGTA194850.041.34778641
TATCTCG194400.041.31619639
CTCGTAT195450.041.1287742
ATCTCGT196450.041.02247640
ACGTCTG206950.040.89796415
CACGTCT208550.040.63813414
GCACACG208850.040.41817511
CGTATGC200300.040.34630644
TCGTATG201750.040.11208743
CACACGT211600.039.9779512
CACTAGC209000.039.44187531
GTATGCC205150.039.414445
GCTTATC208950.038.7191436
GTCACTA213800.038.67212329
Produced by FastQC (version 0.11.5)