FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9943583
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTATG113500.11414396601305586TruSeq Adapter, Index 5 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT17800.034.62701844
TCTCGTA17000.033.34541743
TCGTATG19700.031.23599845
CGTCTGA20950.029.5263116
ATCTCGT19300.029.25505842
ACACGTC21950.028.38624613
ACGTCTG23900.026.25831215
CATCTCG21450.025.90324441
GATCGGA24600.025.4331191
TCGGAAG24650.025.2960823
ATCGGAA24800.025.2341022
CACGTCT26300.024.11881814
CGGAAGA28200.022.4142514
GCACACG30750.020.55530211
CACACGT31050.020.28425612
CATTGGC30150.019.9213835
GTCACAC32150.019.24157329
ATTGGCA33200.018.56555436
GCATCTC30350.018.38089240
TCTGAAC35300.018.2243418
Produced by FastQC (version 0.11.5)