FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15895850
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC635050.3995067895079534TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA82400.041.4571571
ATCGGAA85000.040.391012
ACGGCTA79200.039.8834232
CGGCTAC79400.039.75462333
GTCACGG80300.039.53307329
TCGGAAG86750.039.472473
CGTCTGA85000.039.0411116
ACACGTC86550.038.6538713
CTCGTAT81950.038.51747542
TCTCGTA82550.038.31928341
TCACGGC82200.038.3182230
AGTCACG84050.038.17148628
CGGAAGA89850.038.160674
CGTATGC83250.037.8619544
CATCTCG83450.037.7981739
CACGGCT83400.037.76699431
ACGTCTG88350.037.58624315
ATCTCGT85000.037.21478340
TCGTATG85900.036.8248743
CACGTCT91300.036.56892414
Produced by FastQC (version 0.11.5)