FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9913252
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA11200.026.5962961
CTCGTAT9950.025.5507944
TCTCGTA10300.024.4641343
ATCGGAA13600.022.663692
ATCTCGT11200.022.29738442
ACACGTC13550.022.08316413
CGTCTGA13600.022.00197616
TCGTATG12550.020.97447645
TCGGAAG14700.020.967773
ACGTCTG14700.020.50862115
CACGTCT16000.018.84229514
CGGAAGA18050.016.8269564
AATCTCG16650.015.26909241
CACACGT21050.014.42881412
GCACACG21600.013.95725511
AGTCAAC23650.011.510797534
GTCAACA26850.010.30651935
CAGTCAC29950.010.06640727
CACAGTC28800.09.99927931
CCAGTCA31450.09.72937126
Produced by FastQC (version 0.11.5)