FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18366786
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG186950.10178699746379143TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC29400.035.2779058
CCGATCT29800.034.804389
TTCCGAT32050.032.5716067
GATCGGA33400.032.2286831
CCGTATC28100.032.1060838
TCCGTAT28600.031.70211437
ACGCTCT33500.031.1893841
AGTTCCG28850.031.0374834
CTTCCGA34200.030.7213276
CTCGTAT30000.030.52274144
TATCTCG29550.030.45459741
TCTCGTA30450.030.14555243
CGATCTA11900.029.30447410
ATCGGAA37250.029.1722582
ACACGTC36100.028.9174513
CGTCTGA36850.028.2067916
TCGGAAG38750.027.868833
CGTATCT32300.027.86171739
TCGTATG34500.026.86757545
ATCTCGT34450.026.84126742
Produced by FastQC (version 0.11.5)