FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7933026
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG401330.5058977494842447TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT54000.037.1639644
TCTCGTA54200.037.06833343
GATCGGA62600.036.9459531
TCGGAAG63950.036.201433
TCGTATG56200.035.94933745
ATCGGAA64800.035.8307232
CGTCTGA62750.035.6746916
ATCTCGT56600.035.5362842
ACACGTC63650.035.4883813
CGGAAGA67150.034.6102874
ACGTCTG65900.033.8670315
CACGTCT66800.033.5791414
ACATGTC65350.032.08682332
CAGTCAC69600.031.77670527
CACACGT71750.031.60745212
GCACACG71900.031.60409511
GAAGAGC74150.031.3126226
GTCACAT68650.031.19947629
AGAGCAC74250.031.1189468
AATCTCG64300.031.07083341
Produced by FastQC (version 0.11.5)