FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11520788
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG2254251.9566803937369561TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA269050.043.2155761
ATCGGAA273150.042.607862
TCGGAAG273950.042.4752243
CGTCCGC262950.042.1986333
ACGTCCG263600.042.1969932
GTCCGCA262850.042.18900734
TCCGCAC261950.042.18794635
CGCACAT264800.041.92930637
CCGCACA263750.041.9170936
ACACGTC275050.041.9045513
CGGAAGA278350.041.876544
CGTCTGA274550.041.87433616
AGTCACG268950.041.79404428
GTCACGT268950.041.76749829
ACGTCTG275900.041.6123615
CACGTCC268100.041.5306931
CTCGTAT262550.041.51740644
TCTCGTA262550.041.5088443
CATCTCG263300.041.4247841
ATCTCGT263150.041.42274542
Produced by FastQC (version 0.11.5)