FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9541331
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1301201.3637510322197186TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA166600.040.9524351
ATCGGAA170000.040.2586442
TCGGAAG170050.040.1938863
TCGTATG158700.039.7228245
ACACGTC168450.039.72087513
CGGAAGA172150.039.7035754
AGTCACG165400.039.6920228
CGTCTGA168050.039.6413816
AAACGAT159950.039.6093137
AACGATC158000.039.5997838
GTCACGT165550.039.53333329
TCTCGTA158500.039.4890543
CTCGTAT158800.039.4286244
CACGTGA166300.039.1926931
ACGTCTG170150.039.16534415
CGTGAAA165650.038.9797833
GAAACGA163150.038.9565336
ATCTCGT160950.038.94386342
ACGTGAA167150.038.8857132
CACGTCT171800.038.88086314
Produced by FastQC (version 0.11.5)