FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13980204
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG1062080.7597027911752933TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA134100.041.1599661
ATCGGAA136750.040.422642
TCGGAAG137000.040.36533
TATCTCG129050.040.237241
AGTCACG133450.040.20968228
TCTCGTA129450.040.00844643
CTCGTAT129350.040.00459344
GTCACGT134500.039.69376429
ACACGTC137200.039.61773713
ACGTGGC133300.039.612732
CGTCTGA137800.039.51054416
TCGTATG133100.039.3510645
ATCTCGT131800.039.34644342
CGTGGCC134400.039.05413433
CGGAAGA141950.039.0369534
CACGTCT140150.038.76777614
TCACGTG137400.038.69223830
ACGTCTG141050.038.53635815
CACACGT144800.037.70926712
CACGTGG140450.037.62814731
Produced by FastQC (version 0.11.5)