FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5611814
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC420090.7485814747245721TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA55950.034.33838332
CATCTCG55300.034.2894339
CGGCTAC55900.034.20792833
CTCGTAT56150.034.1709542
TCTCGTA56300.034.0799141
TCGTATG57400.033.5838643
CGTATGC57550.033.57449744
GTCACGG58150.033.11467729
ATCTCGT58550.032.73184640
GTATGCC59300.032.65780345
CGACGCT4650.032.45284335
AGTCACG60450.032.18965528
CACGGCT59850.032.13928631
GCTACAT60250.031.6633835
TCACGGC61150.031.41566830
CTACATC60600.031.29522736
ACATCTC63200.030.03884338
GGCTACA64450.029.73976734
TACATCT65200.029.22546437
GATCGGA71350.028.3996411
Produced by FastQC (version 0.11.5)