FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17552805
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG915360.5214893004280513TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA119050.040.454271
TATCTCG108050.039.99909241
ACACGTC119350.039.47315213
ATCGGAA122850.039.319182
TCGGAAG122700.039.29393
CGTCTGA120700.039.0689316
TCTCGTA111050.038.9995643
CTCGTAT111700.038.75247644
ACGTCTG122500.038.4948615
ATCTCGT112700.038.38865742
CGGAAGA126850.038.1147884
CACGTCT125300.037.5448614
TCGTATG116950.037.32063745
GCACACG129650.036.5628111
CACACGT131950.035.77203412
GTCACAC139450.032.92850529
AGAGCAC152700.031.4709438
CAGTCAC149200.031.11010627
GAGCACA154800.030.9858769
GAAGAGC156450.030.874796
Produced by FastQC (version 0.11.5)