FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12160903
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG1233821.014579262740604TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG161750.040.04460532
TCGTATG159050.039.97616645
GTCCGCA160150.039.96703734
TCCGCAC160050.039.8795535
CGTCCGC162650.039.82302533
CTCGTAT160350.039.66610344
TCTCGTA152000.039.5952343
CGCACAT153000.039.5553937
CCGCACA162350.039.35615536
AGTCACG165100.039.09531428
GTCACGT164900.039.06087529
ATCTCGT154200.039.0449142
CGTCTGA168900.038.8151416
CACGTCC167250.038.75464231
TCACGTC167250.038.68722530
CATCTCG155900.038.5898141
ACACGTC172000.038.4556513
ACGTCTG172500.038.07029315
CACGTCT175700.037.60741414
CACACGT181850.036.42216512
Produced by FastQC (version 0.11.5)