FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9713509
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1014071.0439790605022345TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT127650.037.7264442
TCTCGTA128500.037.4593841
CGTATGC131050.036.95342644
TCGTATG131100.036.9393343
ATCTCGT131350.036.74934440
TCCGATC23900.036.4194958
GATCGGA139900.036.2105641
TTCCGAT24200.035.96827
GTATGCC134700.035.9498745
AATCTCG133700.035.90152739
ATCGGAA144650.035.394682
TCGGAAG145100.035.101933
CGTCTGA143500.034.6862316
ACGCTCT25350.034.6383441
CGGAAGA146950.034.614074
CCGATCT25050.034.567979
CTTCCGA25400.034.4472586
ACACGTC145550.034.13570413
ACGTCTG146850.033.8796415
CACGTCT148100.033.5479514
Produced by FastQC (version 0.11.5)