FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11728826
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG1609321.3721066371007635TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA195350.043.200051
ATCGGAA198300.042.727372
TCGGAAG199500.042.5041963
ACACGTC198600.042.0850813
CGTCTGA198250.042.04589516
TATCTCG188950.042.01973741
CGGAAGA202250.041.959644
CTCGTAT189250.041.79858444
TCGTATG190850.041.79038645
TCTCGTA189700.041.7112943
ATCTCGT191100.041.48812542
ACGTCTG201400.041.35476315
CACGTCT202000.041.254214
GCACACG206700.040.45765311
CACACGT207100.040.3577812
AGAGCAC221650.038.216058
GAAGAGC222650.038.1252486
TCTGAAC219800.037.87351218
GAGCACA223800.037.7684949
CCAGTCA217350.037.66979226
Produced by FastQC (version 0.11.5)