FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7452804
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTATG103900.13941061646059658TruSeq Adapter, Index 9 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT13700.038.09338444
TCGTATG14350.036.9087545
TCTCGTA13600.036.05784243
TATCTCG14950.033.2531841
ACACGTC16700.032.05587813
ATCTCGT15400.031.98935742
CGTCTGA17000.031.62249816
GATCGGA18050.029.9075491
ACGTCTG19200.028.35053615
TCGGAAG19100.028.2924823
CACGTCT19550.027.95803814
ATCGGAA19350.027.9271342
CGGAAGA20250.026.659434
CACACGT22950.023.42407412
GCACACG23850.022.63445511
AAGTATC24650.020.62292938
GTATCTC25700.019.5180540
AGTCACC27700.019.4899528
TCTGAAC28850.019.33552418
CATCAAG28950.018.95934934
Produced by FastQC (version 0.11.5)