FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13479838
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG254960.18914173894374695TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT41100.034.2688744
TCGTATG41750.033.84312445
TCTCGTA38750.033.79236243
GTCACGT44400.032.58126429
AGTCACG45000.031.79687928
ATCTCGT41550.031.6775942
AACGATC42850.030.81984338
AAACGAT43600.030.44471437
CACGTGA47600.030.3441131
CGTGAAA47600.030.29684633
CGTCTGA49000.030.11949516
ACACGTC50550.029.50748813
TCACGTG49050.029.49273930
ACGTGAA49000.029.43122332
ACGTCTG51950.028.6256815
GAAACGA49800.028.59701736
TGAAACG50200.028.23470135
CACGTCT53800.027.76679214
TACGGCG3950.026.7695266
ACGATCT49600.026.58005539
Produced by FastQC (version 0.11.5)