FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16811035
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC2904091.7274903062185047TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA353250.042.4715921
ATCGGAA357400.041.862692
ACACGTC353450.041.617613
TCGGAAG359950.041.5286253
CGTCTGA354050.041.44539616
CGGAAGA360350.041.271844
ACGTCTG358400.040.9423615
TCACGAT343350.040.88934735
TCTCGTA341300.040.87773541
CTCGTAT341850.040.798842
CACGTCT360800.040.6637814
ATCTCGT342800.040.6529240
CATCACG348650.040.50008433
GCACACG364550.040.38743611
CACACGT364600.040.3325312
CGTATGC347400.040.24415644
TCGTATG349250.040.08251643
GTATGCC351100.039.8136445
ACGATCT353500.039.3206937
CACGATC354350.039.23906736
Produced by FastQC (version 0.11.5)