FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17117856
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGT21100.029.64284533
CGATGTA21100.029.53621734
CACCGAT21350.029.506531
ACCGATG21200.029.39689432
GTCACCG23400.027.59448829
TCTCGTA22400.027.3198641
TATCTCG22400.027.01853839
CTCGTAT22800.026.93924142
CGTATGC23700.025.82129944
GATCGGA27700.025.7976511
CGTCTGA28900.024.05564116
ATCTCGT25750.023.6782540
TCGTATG25850.023.58665343
ACACGTC29800.023.32912813
TCACCGA28100.023.13923830
ATCGGAA30900.023.0811312
TCGGAAG31300.022.8580463
ACGTCTG32150.022.04377215
CGGAAGA34200.021.1836134
CACGTCT32950.021.09887914
Produced by FastQC (version 0.11.5)