FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11059207
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG399890.36159012124467876TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG56800.037.78745733
TCGTATG55850.037.30346745
TCTCGTA53000.037.18686743
CTCGTAT56150.037.14422644
TTCGGAA56400.036.9784136
TCGGAAT53500.036.922137
GTTTCGG56850.036.8835834
GTCACGT58150.036.87129
AGTCACG58600.036.54946528
TTTCGGA58000.036.42379835
ATCTCGT55450.035.5032342
TCACGTT60700.035.17395430
CGTCTGA63250.034.75167516
ACACGTC64650.034.0687213
ACGTTTC63250.033.969632
CGGAATC58300.033.88189338
CACGTTT63400.033.74728831
ACGTCTG65450.033.7210515
CACGTCT68550.032.19610214
CACACGT73550.030.34385312
Produced by FastQC (version 0.11.5)