FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12309362
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG1223440.9939101636624221TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG10200.041.245946
CGTACGT157550.040.16998334
CCGTACG159300.040.11002333
ACCGTAC160300.039.9019132
TCGTATG158100.039.83230645
GTACGTA159000.039.64800335
CTCGTAT158950.039.52021844
GTCACCG161900.039.4371329
TCTCGTA150300.039.41452843
CGTAATC149850.039.1856538
ACGGCGA10800.039.162817
CACCGTA163100.039.13413231
TACGTAA160400.038.85310436
ACGTAAT151450.038.83156237
CGTCTGA169850.038.47869516
ATCTCGT154050.038.4550742
ACACGTC173500.037.83777213
ACGTCTG174000.037.63853515
CGGCGAC11400.037.101618
CACGTCT177500.036.90904214
Produced by FastQC (version 0.11.5)