FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17195905
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAG10350.032.60386733
ACGACTA11500.029.73541832
CTCGTAT13800.025.5912844
GATCGGA14800.024.1620241
TCTCGTA13350.023.75796943
TCACGAC14750.023.64297130
GTCACGA15100.023.09495529
TCGTATG16200.022.24558445
CACGACT15650.022.13922731
ATCGGAA17000.020.9315552
ATCTCGT15250.020.50277542
AGTCACG18550.019.28504428
CGTCTGA18700.019.2455216
ACACGTC18550.019.0373713
TCGGAAG19250.018.4838263
ACGTCTG22900.015.9122215
CGGAAGA23500.015.1232644
GACTAGT24050.014.499228534
CACGTCT25250.014.25311814
CACACGT30050.012.79975412
Produced by FastQC (version 0.11.5)