FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14875045
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG537150.36110815126945833TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT71750.037.15884844
TCTCGTA68150.037.04186243
TCGTATG72200.036.98957445
CGACGCT25700.036.94211621
ACGACGC26200.036.49472420
AGTCACG75400.035.7758828
GTCACGA75500.035.609329
TATCTCG70850.035.43899541
CACGACG27050.035.43111419
CGTCTGA78550.035.22908416
ACACGTC79700.034.9465913
ATCTCGT73700.034.2829442
ACACGAC28300.033.78663618
GATCGGA85800.033.6637081
ACGAGTG80800.033.3851432
TACACGA28850.033.2984917
GACGCTC29100.033.1670422
ACGTCTG83900.033.1167315
ATCGGAA87750.033.084992
CACGAGT81600.032.9199831
Produced by FastQC (version 0.11.5)