FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8989446
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2210442.45892794728396TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA259650.043.5032421
ATCGGAA263450.042.836892
ACACGTC259900.042.79872513
CGTCTGA259650.042.7359516
TCGGAAG263750.042.7113953
CGGAAGA264150.042.4773144
ACGTCTG261900.042.39457715
CACGTCT263300.042.09225514
TCTCGTA253000.041.9917641
CTCGTAT253250.041.96807542
GCACACG266150.041.77677511
ATCTCGT253500.041.77580640
CACACGT266450.041.74662412
CGTATGC259100.041.22891644
GTATGCC259000.041.21877345
TCGTATG259950.041.11141643
AATCTCG259000.040.86261739
AGAGCAC275550.040.483168
GAAGAGC277200.040.412646
AGTCACC268950.040.2468728
Produced by FastQC (version 0.11.5)