FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5506663
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC74350.1350182497094883TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA14350.034.40421
CTCGTAT13300.034.0015942
CGTATGC13600.033.08612444
TCTCGTA13600.032.92069641
ACACGTC14850.032.5736813
CGTCTGA14800.032.5317116
ATCGGAA15450.032.0367932
TCGGAAG15700.031.383353
ATCTCGT14600.030.81995240
TCGTATG14700.030.61029243
ACGTCTG16600.029.13970815
CGGAAGA17300.028.8715174
CACGTCT17100.028.41923914
AATCTCG16350.027.79639239
GCACACG19050.025.98259711
GTATGCC17550.025.6393945
CACACGT19650.025.0747412
ACTGACC19450.023.3661232
ACCAATC21100.021.21902536
GACCAAT22300.020.78342835
Produced by FastQC (version 0.11.5)