FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6529077
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC66540.10191333323224708TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGT10250.035.55969233
CGATGTA10150.035.466734
ACCGATG10550.034.7617832
GTCACCG11400.032.56461729
CACCGAT11550.032.141731
TCTCGTA10950.032.0536441
TATCTCG11000.031.9079439
CGTATGC11250.031.59910244
CTCGTAT11200.031.33839642
ATCTCGT12000.029.43643840
TCGTATG12200.029.13851743
TCACCGA13200.028.63533230
CGTCTGA13850.027.77878216
ACACGTC15100.025.33021213
ACGTCTG15500.024.82168615
GTATGCC15350.023.89182945
CACGTCT16800.022.76703614
GATCGGA17050.022.5862711
ATCGGAA17400.022.1112732
TCGGAAG18200.021.2629683
Produced by FastQC (version 0.11.5)