FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10159848
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG574840.5657958662373689TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT69000.039.9104744
TCTCGTA69800.039.51750643
TCGTATG70750.039.11408645
ATCTCGT72100.038.2256942
CGTCTGA78050.037.4445516
GATCGGA80050.036.997971
ACACGTC78800.036.97395313
ATCGGAA81800.036.2230382
TCGGAAG82400.035.8500563
CGGAAGA82900.035.5270234
ACGTCTG83200.035.1538115
CACGTCT84150.034.59652714
GCACACG88100.032.96876511
AATCTCG83800.032.9155441
CACACGT89100.032.6492512
ACATGTC89350.032.20569232
CCAGTCA89750.032.0142326
GTCACAT90550.031.80342529
CAGTCAC91300.031.6923627
AGAGCAC94500.031.1184188
Produced by FastQC (version 0.11.5)