FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6859233
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC216430.3155309055691795TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA30300.041.4781
ATCGGAA31500.040.4265372
TCGGAAG32000.039.654253
ACACGTC31850.039.34652713
CGGAAGA32500.038.9082874
TCTCGTA29900.038.82747741
CGTCTGA32100.038.75972716
CTCGTAT30100.038.56976742
CGTATGC30400.038.26315344
ACGTCTG33300.037.43054615
CACGTCT33750.036.9981414
TCGTATG32050.036.2932943
ATCTCGT32200.036.26370240
AATCTCG32950.035.43827439
GCACACG35800.035.0052211
GTATGCC34500.034.10722745
CACACGT37050.033.94565612
GTCACAC41000.029.90702829
GAGCACA45350.027.8835569
CCAGTCA44950.027.78129226
Produced by FastQC (version 0.11.5)