FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11419109
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC236410.207030163211508TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA39350.034.6023981
TCTCGTA36550.033.67193641
CTCGTAT37400.032.90666642
CGTATGC38000.032.74219544
ATCGGAA42700.032.203932
ACACGTC41750.032.01049413
CGTCTGA41900.032.00329216
ATCTCGT38950.031.65492240
TCGGAAG43450.031.5898913
TCGTATG40100.030.91543843
CGGAAGA44500.030.8445134
ACGTCTG44650.029.98181715
CACGTCT45750.029.3101214
GCACACG47750.028.17670811
GTATGCC46100.027.08660145
CACACGT49650.026.96250212
GATCTCG51750.024.04268339
GTCACAC54100.023.24967829
AGAGCAC64100.022.007568
CCAGTCA60650.021.74048226
Produced by FastQC (version 0.11.5)