FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11402599
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG1085170.9516865409368512TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA144000.041.2283821
ATCGGAA149500.039.9370772
TCGGAAG150300.039.6123923
CGGAAGA150650.039.3710024
AGTCACG144500.039.03894828
TATCTCG139150.039.03175441
CTCGTAT140050.038.9737144
GTCACGT144300.038.96813629
TCTCGTA139800.038.9146543
CGTCTGA149900.038.9041116
TCGTATG141400.038.8243745
ACACGTC151250.038.72049313
CGTGGCC142350.038.69154433
ACGTGGC144950.038.4478332
ATCTCGT142250.038.30768242
TCACGTG146800.038.1665630
ACGTCTG153150.038.10790615
CACGTCT154800.037.81799314
CACGTGG150100.037.2935531
GGCCTTA148700.037.23597736
Produced by FastQC (version 0.11.5)