FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6788905
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTATG598770.8819831769630005TruSeq Adapter, Index 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT69800.042.50856844
TCTCGTA65700.042.18251443
GTCACCG71250.041.73789229
CGCTGAT71700.041.57005734
CACCGCT71900.041.51791831
ACCGCTG72050.041.46270432
TCGTATG72350.041.27410545
CCGCTGA72300.041.25710333
ATCTCGT67350.041.2158942
CGTCTGA73550.041.13401816
ACACGTC75300.040.2679613
CATCTCG69400.039.9335941
TCACCGC75050.039.6859930
GATCGGA77250.039.4957771
TCGGAAG77550.039.4262923
ACGTCTG76850.039.4262315
ATCGGAA78450.039.0603682
CACGTCT77600.039.04546414
GCTGATC77500.038.51706335
CGGAAGA79600.038.4043774
Produced by FastQC (version 0.11.5)