FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14099947
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT26500.023.60180344
TCTCGTA25750.023.15340843
CGACGCT6850.020.6969935
ATCTCGT29500.020.43897242
TCGTATG31800.019.73891845
ACGACGC7700.018.11485734
CGTCTGA38200.017.54784216
ACACGTC40550.016.80825213
GCGACCA8450.016.7729153
GCTAATC35650.016.6646238
ACACGAC8400.016.33699632
TACGGCG1803.936293E-916.2455336
CACGACG8550.016.05083733
TCCGATC8050.015.930346544
TACACGA9200.015.65480931
ACGTCTG44000.015.49033315
TCGGAAG44050.015.3214553
CGACCAC9400.015.0772994
CACGTCT46050.014.89844814
CTACACG9700.014.84786130
Produced by FastQC (version 0.11.5)