FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5187202
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTATG550761.0617670181342467TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT67150.042.20940844
ACACGTC68500.042.13081713
CGTCTGA69000.041.8907216
TCTCGTA61850.041.8256743
TCGTATG68700.041.6936345
ATCTCGT63200.040.86105342
ACGTCTG71150.040.59325815
GATCGGA72800.040.1636771
ATCGGAA73350.040.025722
TCGGAAG73200.040.014313
CACGTCT72500.039.86841214
GCTAATC64550.039.8303238
CACACGT73250.039.42949312
CGGAAGA74300.039.3298074
GCACACG74350.038.96715511
GTCACAC73400.038.8604629
TCTGAAC76000.037.85478618
AGAGCAC77350.037.717558
AATCTCG68750.037.6933341
ACAAGCT75450.037.6257234
Produced by FastQC (version 0.11.5)