FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12231433
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT14600.032.3592344
TCTCGTA14050.031.54435543
TACGGCG1600.025.3064356
TCGTATG19000.025.22073645
ACACGTC20550.024.62953813
ATCTCGT18250.024.40810442
CGTCTGA21500.023.75051116
CATCTCG22300.019.37389241
GATCGGA26200.018.7857721
ACGTCTG27150.018.72510115
ATCGGAA27050.018.7136142
CACGTCT27600.018.50130514
TCGGAAG27500.018.48923
GTAGCCA26500.017.49231136
CGGAAGA29850.017.2591484
TCTGAAC30800.016.5075418
AGTCACC29900.015.73008728
CACACGT33000.015.40565212
CAGTCAC36050.014.35749227
GTCACCT33900.013.940412529
Produced by FastQC (version 0.11.5)