FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23593017
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC315920.13390402761969783TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT63200.024.73404542
TCTCGTA64200.024.31374541
CGTATGC65600.023.96638944
TCGTATG68850.023.03108643
ATCTCGT69800.022.39529640
GTATGCC79700.019.98048645
GATCGGA83600.019.9765971
CGTCTGA89900.019.04267116
TATGCCG25850.018.5331945
ACACGTC95400.018.06226513
ATCGGAA96250.017.842172
ACGTCTG95950.017.8419615
TCGGAAG98000.017.5907823
CACGTCT101200.017.07153314
CGGAAGA103650.016.6964024
GTCACAC101900.015.917129
CACACGT110750.015.64007612
GCACACG111450.015.51935611
GATCTCG105000.015.20885439
CCAGTCA123550.014.00265626
Produced by FastQC (version 0.11.5)