FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5710425
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC646311.131807177224112TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA80550.042.308181
ATCGGAA81750.041.7505072
TCGGAAG81500.041.6025123
CGGAAGA81650.041.3625644
CGTCTGA81150.041.31061616
TCTCGTA78500.041.2721241
CATCTCG78700.041.19582439
ACACGTC81650.041.1954213
CTCGTAT78900.041.0628842
CGTATGC79250.040.99508744
ATCTCGT79500.040.89447440
ACGTCTG82750.040.64780815
CACGTCT83200.040.48204414
TCGTATG80600.040.3363643
GTATGCC81300.039.8783745
GCACACG85100.039.6046511
CACACGT86600.038.89267712
GATCATC85650.038.01063536
CCAGATC87650.037.5796833
AGAGCAC90800.037.0953038
Produced by FastQC (version 0.11.5)