FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7821604
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC370800.4740715587237605TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA48350.041.1343881
ATCGGAA49650.040.3755722
TCGGAAG49950.039.727693
CGGAAGA49850.039.224424
CGATCAG48150.039.0166933
GTCACGA48400.038.67570529
ACACGTC50400.038.3908913
TCTCGTA46900.038.2336541
CTCGTAT47000.038.1051642
TCACGAT49450.037.8999830
ACGATCA49750.037.7166632
CGTATGC47700.037.6874744
AGTCACG49850.037.41628628
CGTCTGA51450.037.38875216
ACGTCTG51900.037.23797215
CACGTCT52650.036.79298414
ATCTCGT48700.036.77429240
TCGTATG49400.036.5271743
CACGATC52050.036.05002631
GTATGCC51500.034.9066545
Produced by FastQC (version 0.11.5)