FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13740023
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG2713231.9746910176205674TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA323200.043.1821821
ATCGGAA327150.042.5422252
TCGGAAG327150.042.452823
CGGAAGA330250.041.8646434
ACACGTC329100.041.6953813
TCGTATG316750.041.60230645
TATCTCG314250.041.59677541
CGTCTGA330100.041.5145516
CTCGTAT315000.041.5120244
TCTCGTA316050.041.36698543
ATCTCGT316800.041.27615742
ACGTCTG332900.041.23971615
CACGTCT333650.041.0256314
GCACACG336450.040.7377111
CACACGT338750.040.4544712
GAAGAGC352050.039.1636056
AGAGCAC351450.039.019218
GAGCACA353950.038.8008239
CAGTCAC348150.038.62690427
TCTGAAC353300.038.5864718
Produced by FastQC (version 0.11.5)