FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14827442
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG543680.3666714730700009TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA69650.039.7385371
ATCGGAA71800.038.41692
CTCGTAT68150.037.86638644
TCTCGTA68300.037.6514643
TCGGAAG73000.037.631293
AGTCACG69550.037.59078628
TATCTCG68600.037.45400641
GTCACGT70350.037.3218429
CGTGGCC70650.036.8130633
ACACGTC72750.036.61634413
CGTCTGA73400.036.3533916
TCGTATG71350.036.262745
CGGAAGA76050.036.0647354
ACGTGGC73150.035.80098332
ATCTCGT71950.035.6788742
TCACGTG74600.035.16543630
ACGTCTG77000.034.79984715
CACGTCT77150.034.46972314
CACGTGG77400.033.9514431
GCACACG81400.032.91877411
Produced by FastQC (version 0.11.5)