FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17126871
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG1203870.7029129839303396TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA154850.041.139561
TCTCGTA147350.040.6307343
CTCGTAT147350.040.6154644
TATCTCG147750.040.49027641
ATCGGAA158150.040.3738822
TCGGAAG158850.040.0968253
ACACGTC157400.040.008813
CGTCTGA157150.039.94359616
TCGTATG150950.039.78096445
ATCTCGT150750.039.7292742
ACGTCTG161150.039.0638215
CGGAAGA162450.038.9877974
CACGTCT162650.038.675914
GCACACG168000.037.59156811
CACACGT170000.037.17578512
GTCACAC176150.034.8690329
AGAGCAC186450.033.9813238
CCAGTCA181950.033.96861626
CAGTCAC183300.033.57114427
GAGCACA190250.033.4090239
Produced by FastQC (version 0.11.5)