FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7965972
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9600.029.0961061
CTCGTAT9000.028.24858342
CGTATGC9100.027.44367644
TCTCGTA9350.027.19097741
ATCGGAA10700.026.2835622
TCGGAAG11150.025.4245743
ATCTCGT9950.025.32520340
TCGTATG9950.024.87312343
ACACGTC11800.023.64273313
CGGAAGA12550.023.127784
CGTCTGA12550.022.76763716
ACGTCTG12750.021.88111915
CACGTCT14300.019.1947214
GTATGCC14450.017.5942745
GCACACG16350.017.47607611
CACACGT18850.015.39700812
GATCTCG17700.015.12626439
GTCACAC19250.013.90830529
CAGTCAC25350.011.44999127
CCAGTCA26350.011.10091826
Produced by FastQC (version 0.11.5)