FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16770552
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1386190.826561940239057TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA178050.041.362881
CGCCAAT177600.038.7773833
ATCGGAA192450.038.4547042
GTCACGC180500.038.3829729
ACGCCAA180150.038.2792532
TCGGAAG192950.038.1933863
TATCTCG178900.038.14358539
AGTCACG182950.038.13976328
CTCGTAT179950.037.89601542
ACACGTC190200.037.88825213
TCTCGTA180300.037.87236441
CGTCTGA189850.037.85144416
CGGAAGA196250.037.470894
CGTATGC182550.037.45487244
ACGTCTG193750.037.11275515
ATCTCGT184300.037.0992240
TCGTATG185000.037.05613743
CACGTCT194650.037.0220714
GCACACG199700.036.35624711
CACACGT202100.035.87997412