FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15979273
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1658341.0378069139941473TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA208300.041.7622641
ATCGGAA214050.040.8714832
TCGGAAG214800.040.5302733
CGGAAGA218750.039.7264064
ACACGTC217000.039.40918713
CGTCTGA217400.039.28493516
CTCGTAT205900.039.23941442
TCTCGTA206550.039.08325241
CATCTCG207350.038.9107639
ATCTCGT209300.038.72022640
CGTATGC209250.038.708144
ACGTCTG221500.038.58823415
CACGTCT221800.038.53604514
TCGTATG211850.038.28602243
GTATGCC214750.037.8212745
GCACACG227850.037.70042811
CACACGT228300.037.5768412
CCAGATC228800.035.74437733
GATCATC228150.035.6292636
AGTCACC233300.035.57924728
Produced by FastQC (version 0.11.5)