FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12710482
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG262690.20667194210258902TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT34200.036.31382444
TCTCGTA34800.035.75237743
TCGTATG36050.035.01197445
TATCTCG36250.034.32227741
GTCACGA36750.034.0388429
ATCTCGT37800.033.0934542
AGTCACG39000.032.30708728
ACGAGTG40850.030.89808532
CACGAGT41550.030.26923831
ACACGTC44450.029.5087413
CGTCTGA43850.029.50204716
ACGTCTG48450.026.88677215
CACGTCT50650.025.94103414
GGATATC51500.024.37732138
GATCGGA54800.024.183841
ATCGGAA55450.023.938912
TCGGAAG55750.023.9311583
CACACGT55650.023.89330312
CGGAAGA56250.023.519564
CGAGTGG54200.023.45361933
Produced by FastQC (version 0.11.5)