FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5295853
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG225830.4264279994176575TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGA28800.039.5298529
TATCTCG28350.039.2049641
CTCGTAT28700.038.6488244
TCTCGTA28850.038.3698943
CGTCTGA30700.038.3292616
TCGTATG29050.038.26062445
ACACGTC31150.037.99223313
ATCTCGT29500.037.90579242
AGTCACG30250.037.85851728
GATCGGA31650.037.572881
CACGAGT31050.036.81028731
ACGAGTG30900.036.77054232
ATCGGAA32600.036.7164882
TCGGAAG32650.036.6602633
CACGTCT32300.036.6395714
ACGTCTG32450.036.40086715
CGGAAGA34350.034.848234
GCACACG34500.034.49877511
CACACGT34950.033.92583512
TCACGAG34150.033.53468330
Produced by FastQC (version 0.11.5)