FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10213523
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCATCTCGTATGC1078941.056383776685087TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCATCTCGTATG171770.16817899171519954TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC132300.042.37610245
ATCGGAA152250.037.666951
CTCGTAT147350.037.55877743
CGTCTGA150650.037.5109315
ACACGTC152000.037.33984412
TCGGAAG153450.037.3283462
TCTCGTA133500.037.2760942
TCGTATG150100.037.14044644
ACGTCTG152900.037.0030814
CACGTCT154100.036.83171513
ATCTCGT135700.036.6883441
CATCTCG136600.036.4774140
CGGAAGA157500.036.3684773
GCACACG159750.035.66918610
CACACGT160400.035.51061211
GCCATGC161900.034.61158434
AGAGCAC166500.034.3714187
GCATCTC146200.034.06678839
CCAGTCA166650.033.8161925
GAGCACA169450.033.7730378
Produced by FastQC (version 0.11.5)