FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4722183
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA5050.035.64198732
CGGCTAC5400.033.3318633
GTCACGG5700.031.5775529
TCACGGC6050.029.75075130
CTCGTAT6100.029.13929242
AGTCACG6350.029.05475828
TCTCGTA6200.029.03097341
CGTATGC6400.028.4765144
CGTCTGA7350.027.549816
CACGGCT6550.027.47969831
ATCTCGT6700.027.20028940
ACACGTC7500.026.99880613
TCGTATG6850.025.94885443
CATCTCG7450.024.46200639
GATCGGA8550.023.4426041
ATCGGAA8850.023.134572
TCGGAAG8950.022.6246993
ACGTCTG9050.022.37469915
CACGTCT9400.022.25965314
CGGAAGA9750.021.0004064
Produced by FastQC (version 0.11.5)