FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4216462
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG4800.033.27958733
CTCGTAT5600.032.14124744
TTCGGAA4900.031.68208936
GATCGGA6600.030.3768521
TCGGAAT5200.030.28694537
GTTTCGG5250.029.99849934
TCTCGTA6050.029.75057643
ACACGTC6900.028.69421613
CGTCTGA7000.028.60571116
TTTCGGA5500.028.22585935
AGTCACG6200.027.94281228
GTCACGT5900.027.83759129
ATCTCGT6500.027.69092242
TCGGAAG7500.026.9986483
ATCGGAA7600.026.6434022
TCGTATG6800.026.13839545
ACGTCTG7700.025.42080315
CGGAAGA8250.024.0005054
CACGTCT8350.023.71138814
ACGTTTC7000.023.14169932
Produced by FastQC (version 0.11.5)