FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4762464
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA4750.026.5252743
CTCGTAT4900.025.25410744
CGTCCCG1056.121263E-821.42750235
ATCTCGT6500.019.38385242
GTCACGT5500.019.22631329
TATCTCG5050.018.71193541
TCGTATG6800.018.52868345
AGTCACG5900.018.3049428
CGTCTGA8300.017.07746116
ACGTCTG9100.015.57614615
CCCGATC1602.970355E-715.46797738
ACACGTC9100.015.32890513
ACGTGGC6650.014.88647632
CGTGGCC6350.014.88114633
CACGTCT9800.014.69314414
GTCCCGA1705.9022204E-714.55809636
TCACGTG6900.014.34711130
TATGCCG1758.187744E-714.142342
ATCGGAA10500.013.4993262
TCGGAAG10650.013.3091953
Produced by FastQC (version 0.11.5)