FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4849376
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG310740.6407834739974793TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT37550.040.62376844
CGTCCGC38100.040.450733
ACGTCCG38300.040.415732
TCTCGTA37800.040.41460843
CGCACAT37800.040.29556737
GTCCGCA38300.040.23946834
TCGTATG38100.040.03733445
TCCGCAC38300.039.76951635
CCGCACA38400.039.72454536
GTCACGT39550.039.36588729
ATCTCGT38850.039.32232342
AGTCACG39900.039.02137828
CATCTCG39150.038.96353541
CACGTCC40100.038.6576431
TCACGTC40800.038.38039830
CGTCTGA44250.035.74394216
ACACGTC44300.035.703613
ACGTCTG45550.034.7238115
CACGTCT45900.034.45903414
GCACATC45100.033.82311238