Basic Statistics
Measure | Value |
---|---|
Filename | EGAF00005727704 |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 4849376 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG | 31074 | 0.6407834739974793 | TruSeq Adapter, Index 18 (97% over 40bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 3755 | 0.0 | 40.623768 | 44 |
CGTCCGC | 3810 | 0.0 | 40.4507 | 33 |
ACGTCCG | 3830 | 0.0 | 40.4157 | 32 |
TCTCGTA | 3780 | 0.0 | 40.414608 | 43 |
CGCACAT | 3780 | 0.0 | 40.295567 | 37 |
GTCCGCA | 3830 | 0.0 | 40.239468 | 34 |
TCGTATG | 3810 | 0.0 | 40.037334 | 45 |
TCCGCAC | 3830 | 0.0 | 39.769516 | 35 |
CCGCACA | 3840 | 0.0 | 39.724545 | 36 |
GTCACGT | 3955 | 0.0 | 39.365887 | 29 |
ATCTCGT | 3885 | 0.0 | 39.322323 | 42 |
AGTCACG | 3990 | 0.0 | 39.021378 | 28 |
CATCTCG | 3915 | 0.0 | 38.963535 | 41 |
CACGTCC | 4010 | 0.0 | 38.65764 | 31 |
TCACGTC | 4080 | 0.0 | 38.380398 | 30 |
CGTCTGA | 4425 | 0.0 | 35.743942 | 16 |
ACACGTC | 4430 | 0.0 | 35.7036 | 13 |
ACGTCTG | 4555 | 0.0 | 34.72381 | 15 |
CACGTCT | 4590 | 0.0 | 34.459034 | 14 |
GCACATC | 4510 | 0.0 | 33.823112 | 38 |