FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14164276
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1684901.1895419151674254TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT204750.040.9655142
TCTCGTA206050.040.70705441
ATCTCGT207300.040.45074540
CGTCTGA221000.040.09099216
CGTATGC209600.040.0712744
GATCGGA225600.039.9951671
AATCTCG209800.039.93655439
TCGTATG210900.039.90961543
ACACGTC223650.039.83727613
ACGTCTG224950.039.4670315
ATCGGAA229750.039.448782
TCGGAAG230100.039.350123
CACGTCT226900.039.2567514
CGGAAGA230550.039.136684
GTATGCC217300.038.76497345
GCACACG234450.038.2228811
CACACGT235650.038.00914412
AGTCACC230900.037.55700728
GTCACCT230750.037.37651429
CCAGTCA239200.036.61127526
Produced by FastQC (version 0.11.5)